Application

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Urease Buffer Reagent has been used: to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside methodin cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cellsto study the metabolic capacity of rat hepatocytes

General description

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.

Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants.Detection of urease activity is a potential diagnostic tool for Helicobacter pyloriinfections and several oral bacterial infections. two Ni²⁺ions bridged by a hydroxyl group and a carbamylated lysine.