Application

Trizma has been used:As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cellsAs a component of pre-hybridization buffer in in situ hybridizationTo suspend dried protein before SDS-PAGE protein characterizationAs a component of lysis buffer to wash and lyse cells before immunoprecipitationIn the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresisIn studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLCIn capillary electrochromatography and UV analysis of tocopherols and tocotrienols

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Easily compare specifications for Trizma products with the Trizma specification table.

Packaging

5 kg in poly drum

1 kg in poly bottle

100, 500 g in poly bottle

T6066-1KT:Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.