Application

Anti-Rabbit IgG (H+L)-Biotin antibody produced in chicken has been used in immunohistochemistry.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody offers protection against bacterial, fungal and viral infections. IgG antibody share similar function with IgM antibody in complement system activation. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. IgG mediates hypersensitivity type II and type III. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

General description

IgG (immunoglobulin G) consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. The chains are interlinked with a disulfide bond. Both the L and the H chains consist of a variable domain at the N-terminal. The C-terminal bears′ constant domains: three in H chain with a hinge region and one in the L chain. IgG antibody is enriched in mother′s milk. IgG contributes to 10−20% of plasma protein and is regarded as one of the most abundant serum protein.

Immunogen

Rabbit IgG whole molecule

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Physical properties

Antibody format: IgG

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Chicken Serum, Rabbit IgG and Rabbit Serum.