Application

Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:500-1:3000, IHC-Wm: 1:50-1:500, Immunostaining: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Anti-PCNA antibody has been used in the detection of proliferating cells by immunofluorescence.

Biochem/physiol Actions

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Proliferating cell nuclear antigen (PCNA) controls the production of the leading and lagging strands, which are required for the duplication of DNA. It acts as a cell cycle regulatory protein. This protein regulates apoptosis. PCNA is also involved in non-replicative DNA synthesis events.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

Proliferating cell nuclear antigen (PCNA) is a ring-shaped homotrimer protein, that is located at the center of the faithful duplication of eukaryotic genomes. Since it encircles the DNA, PCNA is also known as a sliding clamp. This gene is mapped to human chromosome 20p12.3.

Immunogen

Recombinant protein containing a sequence corresponding to a region within amino acids 31 and 222 of pcna according to NP_571479

Physical form

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.