Application

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis. Fraction 1: Soluble/Cytoplasmic Proteins Fraction 2: Membrane ProteinsThe special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Features and Benefits

Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels. Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis. Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution. Pre-weighed dry blends - Stable and easy to reconstitute Conveniently Packaged - No waste. Use only the amount needed.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC