Application

ProteoPrep^® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.

Disclaimer

Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.

Features and Benefits

Four pre-mixed solubilization reagents - Enables rapid solubilization. Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution. Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.

General description

ProteoPrep^® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

The ProteoPrep^® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists. This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.

Storage and Stability

These reagents should remain stable for at least 6 months in their unopened container.