General description

The Plant Hydrophilic Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all hydrophilic proteins from any type of plant species or tissue. The kit includes two reagents, a plant specific protease inhibitor cocktail and a specially formulated reagent to extract hydrophilic proteins (Plant Protein Extraction Reagent Type 1). The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases, and aminopeptidases. It contains pepstatin A, E-64, leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, and 1,10-phenanthroline. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process. Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the protein extraction reagent provided. Plant debris is pelleted by centrifugation and the hydrophilic protein extract is collected. The end result is a qualitative soluble hydrophilic protein sample, ready for downstream proteomic analysis.

Quantity

Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250 mg