Application

Pectolyase fromAspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Packaging

1 g in poly bottle

100, 250 mg in poly bottle

Unit Definition

One unit will liberate 1.0 µmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.