Features and Benefits
Features and Benefits• Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs• Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence • No self-complementarity or primer dimer artifacts with the Universal PCR Primer• Optimized primer Tm designed to match the Universal PCR Primer• Universal cycling conditions ensures robust amplification for all assays in profiling experiments• Optimized PCR product Tm (75-78° C)
The MystiCq microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq microRNA SYBR Green qPCR ReadyMix on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.
MystiCq is a registered trademark of Qiagen Beverly Inc.
Formerly hsa-miR-125b. Compatible with mmu-miR-125b-5p and rno-miR-125b-5p.