Application

Western Blotting Analysis: A 1:100 dilution from a representative lot detected Chikungunya virus in BHK cells infected with CHIKV (Courtesy of an outside independent laboratory).

Immunocytochemistry Analysis: A representative lot detected Chikungunya virus in BHK cells infected with CHIKV (Courtesy of an outside independent laboratory).

Anti-Chikungunya virus, clone 6A11, Cat. No. MABF2051 is a mouse monoclonal antibody that detects E1 protein of Chikungunya virus (CHIKV) and has been tested for use in Immunocytochemistry and Western Blotting.

General description

Chikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. (Ref.: Marsinoul, P., et al. (2014). Virology 464-465; 111-117; Lam, S., et al. (2015). mAbs 7(6), 1178-1194).

Immunogen

CH122508 strain of Chikungunya virus.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .

Specificity

Clone 6A11 specifically detects Chikungunya virus in infected cells.

Target description

~9.17 kDa calculated for E1 protein. Uncharacterized bands may be observed in some lysate(s).