Application

Research CategoryInflammation & Immunology

This mouse monoclonal Anti-Zika Virus, clone ZV-54, Cat. No. MABF2046 is tested for use in Western Blotting, Neutralizing, and Flow Cytometry, for the detection of Zika virus envelope protein.

Flow Cytometry Analysis: A representative lot detected Zika Virus in Flow Cytometry applications (Zhao, H., et. al. (2016). Cell. 166(4):1016-27).

Neutralizing Analysis: A representative neutralized Zika Virus in Neutralizing applications (Zhao, H., et. al. (2016). Cell. 166(4):1016-27).

Neutralizing Analysis: A representative lot detected Zika Virus in ZIKV H/PF/2013 (French Polynesia, 2013) (Courtesy of Dr. Michael Diamond, M.D., Ph.D., Washington University School of Medicine, St. Louis, MO USA).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Genome polyprotein (UniProt: Q32ZE1) from Zika virus strain Mr 766 is a large molecular weight protein. In Zika virus surface proteins are arranged in an icosahedral-like symmetry. Attachment of the viral envelope protein E to host receptors mediates internalization into the host cell by apoptotic mimicry. Following the fusion of virus membrane with host endosomal membrane, RNA genome is released into the cytoplasm. Polyprotein components include Protein C homodimerizes and plays a role in virus budding by binding to membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. The Pr peptide prevents premature fusion activity of the envelope proteins in trans golgi by binding to envelope protein E (at pH 6.0). The PrM peptide serves as a chaperone for envelope protein E during intracellular virion assembly. Polyprotein also has a RNA-directed RNA polymerase NS5 that replicates the viral (+) and (-) genome and methylates viral RNA cap at guanine N-7 and ribose 2 -O positions. The non-structural protein 1 can form homodimers and homohexamers is involved in immune evasion, pathogenesis and viral replication and non-structural protein 2A functions in virion assembly and antagonizes the host immune response. The non-structural protein 2B is essential for the serine protease function of NS3. The serine protease NS3 has three different enzymatic activities - serine protease, NTPase and RNA helicase. The non-structural protein 4A of virus regulates the ATPase activity of the NS3 helicase while the non-structural protein 4B inhibits interferon-induced host STAT1 phosphorylation and its nuclear translocation. The 2k peptide serves as a signal peptide for NS4B and is required for the interferon antagonism. Clone ZV-54 is reported to neutralize infection of African, Asian, and American strains of Zika virus to varying degrees. (Ref.: Zhao, H et al. (2016). Cell 166(4); 1016-27).

Immunogen

Zika virus strains MR-766 and H/PF/2013

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Format: Purified

Quality

Evaluated by Western Blotting in recombinant Zika virus envelope protein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant protein consisting of ectodomain of Zika Virus Envelope.

Specificity

Clone ZV-54 specifically detects Zika virus envelope protein. It binds to the ectodomain III and displays high affinity (Kd = 7.9 nM) for Zika virus.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~50 kDa observed (recombinant fragment); 378.74 kDa calculated for whole protein. Uncharacterized bands may be observed in some lysate(s).