Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Inactivated and purified Rabies Virus (Pasteur virus strain).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Reconstitution

Please refer to lot specific datasheet.

Specificity

Clone D1-25 is a mouse monoclonal antibody that detects Rabies virus glycoprotein. It targets an epitope within nine amino acids in the antigenic site III.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Glycoprotein (UniProt: P08667; also known as RABV glycoprotein) is encoded by the G gene (Gene ID: 1489856) in rabies virus (strain Pasteur vaccins). Rabies virus glycoprotein is a single-pass type I membrane protein that attaches the virus to host cellular receptors, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been reported that the muscular form of nicotinic ACh receptor, p75 neurotrophin receptor (p75NTR) and the neuronal cell adhesion molecule can bind to glycoprotein and facilitate the entry of rabies virus into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524) domain. Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone D1-25 recognizes an epitope in antigenic site III on the rabies virus (RABV) glycoprotein. (Ref.: Nagarajan, T., et al. (2006). Biologicals. 34(1); 21-27; Müller, T., et al. (2009). PLoS Negl. Trop. Dis. 3(11); e542; Sissoëff,1, L., et al. (2005). J. Gen. Virol. 86(9); 2543-2552).