Application

Flow Cytometry Analysis: A representative lot detected Thy 1.1 (CD90) in CAK1.3 and CAK4.4 cell lines (Ogasawara, K., et. al. (1990). Microbiol. Immunol. 34(12):1025-39).

Research CategoryInflammation & Immunology

This mouse monoclonal Anti-Thy 1.1 (CD90), clone T11D7e, Cat. No. MABF1961 is validated for use in Flow Cytometry for the detection of Thy-1 membrane glycoprotein, CD90.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Thy-1 membrane glycoprotein (UniProt: P01830; also known as Thy-1 antigen, CD90) is encoded by the Thy1 (also known as Thy-1) gene (Gene ID: 24832) in rat. Thy-1 is a heavily glycosylated, GPI-anchored membrane glycoprotein that is distributed throughout mammalian species. Thy-1 is synthesized with a 19 amino acid signal sequence and 31 amino acid C-terminal transmembrane domain that is present in pro form, but removed when transferring the 112 amino acid mature peptide to GPI anchor. It is present in large amounts on thymus and brain cells and in smaller quantities on fibroblasts, epidermal cells, mammary glands and immature skeletal muscle. In many of these tissues the level of Thy-1 expression changes dramatically during cell differentiation. Thy-1 plays a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Thy-1 is one of the most heavily glycosylated membrane proteins with a carbohydrate content up to 30% of its molecular mass. In most species Thy-1 has 3 N-glycosylation sites (Asn 23, 74, and 98) but no O-glycosylation. The glycosylation appears to be tissue specific and the composition of Thy-1 carbohydrate moieties is shown to vary between different tissues or even among cells of the same lineage at different stages of differentiation. Thy-1 is a differentiation marker for thymocytes and can serve as a surrogate marker for various kinds of stem cells.

Immunogen

Xenogenic rat thymocytes.

Epitope: extracellular domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified by ion-exchange chromatography

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in rat thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected Thy 1.1 (CD90) in one million rat thymocytes.

Specificity

Clone T11D7e detects Thy-1.1 (CD90) in rat thymocytes.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

18.17 kDa calulated