Analysis Note

ControlHuman Interleukin-1 beta recombinant protein

Application

Anti-Interleukin-1 beta (IL-1B) Antibody, clone 4A7 is an antibody against Interleukin-1 beta (IL-1B) for use in WB.

Luminex Assay Analysis: A previous lot was used by an independent laboratory in Luminex (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX).

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-1 (IL-1) is a cytokine that is thought to be connected to neurodegeneration and in central nervous system-mediated defense reactions to inflammation. Interleukin-1 beta (IL-1B) maintains mitogen-induced proliferation, IL-2 production and expression of IL-2R. IL-1 beta also seems to have the ability to boost the number of cells that move into the G1 phase of the cell cycle. IL-1 beta also has the capacity to stimulate apoptosis on normal human fetal membranes. IL-1 beta may play an important role in inflammation regulating cytokine networks.

Immunogen

Full length recombinant protein corresponding to human Interleukin-1 beta.

Linkage

Replaces: 04-462; MABF22

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in lysate from cells expressing human Interleukin-1 beta recombinant protein.

Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human Interleukin-1 beta recombinant protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed