HPTP1MAG-66K-03

Application

Analytes: ACTH, Agouti-Related Protein (AGRP), CNTF, FSH, GH, LH, TSH Recommended Sample Type: Human serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysates and supernatants Recommended Sample Dilution: 25 µL per well of undiluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media Assay Run Time: Overnight (16-18 hr) at 2-8°C Research Category: Endocrine Research Subcategory: Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Called the “master gland” because it controls many other endocrine glands, the pituitary secretes several key hormones that play important roles in the regulation of metabolism, growth, and reproduction. Piuitary hormones are responsible for the stimulation of the adrenal gland (ACTH), thyroid gland (TSH), ovaries and testes (FSH, LH), as well as blood pressure and the amount of water excreted by the kidneys. Through its connection with the pituitary, the hypothalamus modulates endocrine function by detecting hormone levels. The MILLIPLEX^® Human Pituitary Panel 1 is a 7-plex kit to be used for the simultaneous measurement of any or all of the following analytes in human serum, plasma or cell/tissue culture samples: ACTH, AGRP, CNTF, FSH, GH, LH, and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Endocrine

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

Packaging

96 well plate

Everything you need in a single kit.

Specificity

Cross ReactivtyThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

assay range	standard curve range: 0.039-160 µIU/mL(TSH), standard curve range: 2.4-10,000 pg/mL(AGRP), sensitivity: 0.02 mIU/mL(minDC+2SD; FSH), accuracy: 77%(AGRP), standard curve range: 0.049-200 mIU/mL(LH), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(CNTF), standard curve range: 3.0-12,500 pg/mL(ACTH), sensitivity: 0.02 mIU/mL(minDC+2SD; LH), standard curve range: 2.4-10,000 pg/mL(GH), sensitivity: 2.4 pg/mL(minDC+2SD; ACTH), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(GH), standard curve range: 122-500,000 pg/mL(CNTF), sensitivity: 6.88 pg/mL(minDC+2SD; GH), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(TSH), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(AgRP), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(ACTH), sensitivity: 0.02 µIU/mL(minDC+2SD; TSH), standard curve range: 0.024-100 mIU/mL(FSH), accuracy: 97%(FSH), accuracy: 105%(TSH), sensitivity: 1.12 pg/mL(minDC+2SD; AGRP), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(FSH), inter-assay cv: ﹤15%intra-assay cv: ﹤10%(LH), accuracy: 87%(LH), sensitivity: 84.42 pg/mL(minDC+2SD; CNTF)
detection method	fluorometric (Luminex xMAP)
manufacturer/tradename	Milliplex^®
Quality Level	200
shipped in	wet ice
species reactivity	human
technique(s)	multiplexing: suitable