Application

•Analytes: Antithrombin III, Complement Factor H (CFH), Extracellular matrix Protein 1 (ECM1), Vitamin D binding protein•Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates•Note: Serum has a thrombin/antithrombin III complex bound to vitronectin. To accurately quantify antithrombin III concentrations we recommend using plasma samples because they lack the vitronectin/thrombin/antithrombin complex.•Recommended Sample Dilution: 25 μL of a 1:10,000 dilution of plasma or serum, or 25 μL of tissue/cell culture sample diluted in an appropriate control medium (dilution factors may vary)•Assay Run Time: Overnight (16-18 hours) at 2-8°C•Research Category: Cancer

Components

Human Circulating Cancer Biomarker Panel 2 Standard (HCC-8058-2)Human Circulating Cancer Biomarker Panel 2 Quality Controls 1 and 2 (HCC-6058-2)Set of one 96-Well Plate with 2 SealersBead Diluent (LBD-4)Assay Buffer (L-AB)
Note: Contains 0.08% Sodium Azide10X Wash Buffer (L-WB)
Note: Contains 0.05% ProclinHuman Circulating Cancer Biomarker Panel 2 Detection Antibodies (HCC-1058)Streptavidin-Phycoerythrin (L-SAPE 6)

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology. MILLIPLEX^® Circulating Cancer Biomarker Panel 2 is a 4-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell lysate and culture supernatant samples: Extracellular matrix Protein 1 (ECM1), Antithrombin III, Vitamin D binding protein, and Complement Factor H (CFH). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Circulating Cancer

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.