Application

Immunofluorescence (see comments)

General description

Aqueous mounting media designed to preserve and enhance the labeling of tissue sections or cell preparations fixed on slides. Suitable for many fluorophores including fluorescein, Oregon Green™, Alexa™ 488, rhodamine, Texas Red, Cyanine (Cy™) 3, and Alexa™ 546. Inclusion of DAPI in the Fluorescent Mounting Media allows for the visualization of all cells at an excitation wavelength of 330-380 nm.

An aqueous mounting medium designed to preserve and enhance the labeling of immunoreactivity in tissue sections. Sustains the fluorescent signal from labeled samples on slides. Inclusion of 4,6-diamidino-2-phenylindole (DAPI; Cat. No. 268298) allows for the visualization of unlabeled (non-apoptotic) cells. Suitable for use with a wide variety of fluorophores.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.

Packaging

8 ml in Glass bottle

Physical form

8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)