Analysis Note

Stimulates tyrosine phosphorylation of the ErbB-3/HER-3 receptor.

The biological activity is measured by its ability to activate the c-ErbB3/HER-3 receptor in treated MCF-7 cells.

Biochem/physiol Actions

Growth factor ligand for ErbB3 and ErbB4 receptor tyrosine kinases. The binding of HRG results in receptor dimerization and receptor trans-autophosphorylation. The phosphorylated receptors recruit cellular signaling proteins, initiating signaling pathways.

General description

Truncated human sequence (amino acids 178-241) corresponding to the EGF domain and purified as a GST-fusion protein, cleaved with thrombin.

Physical form

Solution in phosphate buffered saline containing 30% glycerol.