Application

Chicken polyclonal anti-Complement C3 antibody is used to tag complement C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA and western blotting. It is used as a probe to determine the presence and roles of complement C3 as a component of the complement system.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Anti-complement C3 antibody produced in chicken has been used in immunoblot analysis.

Biochem/physiol Actions

Complement factor 3 (C3) plays an essential role in the activation of the complement system. C3-convertase (C4b2a) cleaves C3 complement into C3a and C3b. C3b can bind covalently, through its reactive thioester, to cell surface carbohydrates or immune complexes and involve in the self-activation loop of complement activation via the alternate pathway. In addition, C3b also interacts with C3 convertases (C4b2a or C3bBb) to form the C5 convertase, which cleaves complement factor 5 into C5a and C5b. Derived from proteolytic degradation of complement C3, C3a (anaphylatoxin) facilitates the recruitment of inflammatory cells. C3a stimulates smooth muscle contraction, enhances vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Mutation in C3 gene leads to the development of age-related macular degeneration (AMD).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chicken polyclonal anti-Complement C3 antibody reacts with human complement C3.

The complement factor 3 (C3) gene, with 41 exons spanning 41kb on genomic DNA, is mapped to human chromosome 19p13.3. It is the third component of complement and is characterized with α and β chains. It is one of the most abundant complement protein in the serum.

Complement C3 plays a central role in the activation of the complement system. It’;s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes.

Immunogen

Complement C3 native protein.

Immunogen Sequence: GI # 4557385, sequence 1-1663

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.