Application

Anti-Fibronectin antibody produced in chicken is suitable for western blotting at a working dilution of 1:500 and for cell staining at a working dilution of 1:200. It is also suitable for indirect ELISA.

Biochem/physiol Actions

Fibronectin (FN1) is a glycoprotein that is involved in cell adhesion and migration processes, such as embryogenesis, wound healing, blood coagulation, host defense, and metastasis. It binds to cell surfaces and compounds, such as collagen, fibrin, heparin, fibulin, integrins, cell adhesive mac-2 binding protein (M2BP), cartilage oligomeric matrix protein (COMP), DNA, and actin. Fibronectin interaction with FLRG (Follistatin-related gene protein) and follistatin is important for human hematopoietic cell adhesiveness. It is associated with glomerulopathy with fibronectin deposits, an autosomal dominant disease. Polymorphism in gene is linked with increased risk of knee osteoarthritis. Human pathogens, Staphylococcus aureus and Streptococcus pyogenes, target fibronectin for adhesion to and invasion of host cells. Pigment epithelium-derived factor (PEDF) down-regulates fibronectin to suppress breast cancer metastasis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The gene FN1 (Fibronectin) is mapped to human chromosome 2q34. It is present as a soluble dimeric form in plasma. At the cell surface and in the extracellular matrix, it is present as a dimeric or multimeric form.

Immunogen

Fibronectin native protein.

Immunogen Sequence: GI # 31397, sequence 1-2328

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.