Alkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.

• Bacterial alkaline phosphatase recombinantly expressed in E. coli
• Active in PCR and restriction endonuclease buffers
• Inactivated by heating at 75°C for 5 minutes
• Use together with Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing