Application

Deoxyribonuclease II from bovine spleen has been used: as a component of digestion mix to isolate immune cells from colon samplesto digest retinal explants for single-cell suspension preparationfor isolation of stromal cells from human colon tissuein the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Packaging

30000, 150000, 300000 units in poly bottle

Unit Definition

One Kunitz unit will produce a δA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg²⁺] = 0.83 mM