D1806-250UN Display Image
D1806-250UN Display Image

Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2

Code: D1806-250UN D2-231

Application

Taq DNA Polymerase from Thermus aquaticus has been used:• in the quantification of fungal growth by PCR (polymerase chain reaction) and photometric assay...

read more

£121.00 EACH
List Price

Application

Taq DNA Polymerase from Thermus aquaticus has been used:• in the quantification of fungal growth by PCR (polymerase chain reaction) and photometric assay• in conventional RT(reverse transcriptase)-PCR• in SSR (simple sequence repeats) genotyping

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

• Low per unit cost of Taq

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

featurehotstart: no
Quality Level200
shipped inwet ice
concentration5 units/L
formliquid
Featured IndustryAgriculture
suitabilitysuitable for PCR and automated sequencing reactions
recombinantexpressed in E. coli
mol wt94 kDa
colorcolorless
storage temp.20C

Code
Description
Unit Size
List Price
Qty
D1806-1.5KU
Unit:EACH
List Price: £596.00
Source:List Price
ADD
D1806-10X1.5KU
Unit:EACH
List Price: £5,180.00
Source:List Price
ADD
D1806-20X250UN
Unit:EACH
List Price: £2,160.00
Source:List Price
ADD
D1806-5KU
Unit:EACH
List Price: £1,820.00
Source:List Price
ADD
Cas Number9012-90-2