D1501-50MG Display Image

Deoxyribonucleic acid sodium salt from calf thymus, Type I, fibers

Code: D1501-50MG D2-231

Application

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function....

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£31.70 50MG
List Price

Application

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Packaging

50, 100, 500 mg in poly bottle

1, 5 g in poly bottle

Physical form

fibrous preparation

Physical properties

λmax 259 nm (100 mM phosphate buffer, pH 7.0).DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 µg of double-stranded DNA.

Preparation Note

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.

This product is prepared from calf thymus tissue of unspecified gender.

Quality

Highly Polymerized

Reconstitution

This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (µg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.

Quality Level300
storage temp.2-8C
colorwhite
formfibers
typeType I

Code
Description
Unit Size
List Price
Qty
D1501-100MG
Unit:100MG
List Price: £54.40
Source:List Price
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D1501-1G
Unit:1G
List Price: £228.00
Source:List Price
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D1501-500MG
Unit:500MG
List Price: £219.00
Source:List Price
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D1501-5G
Unit:5G
List Price: £1,040.00
Source:List Price
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Cas Number73049-39-5