D1054-10MG Display Image
D1054-10MG Display Image

Dihydrorhodamine 123, >=95%

Code: D1054-10MG D2-231

Application

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It ...

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£256.00 10MG
List Price

Application

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Biochem/physiol Actions

Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst.

Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa.

General description

Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluorescein (DCDHF), lipophilic and diffuses through cell membrane.

Packaging

2, 10 mg in ampule

Substrates

Peroxidase substrate

Quality Level200
solubilityDMSO: 5 mg/mL
InChI keyFNEZBBILNYNQGC-UHFFFAOYSA-N
assay95%
formpowder
InChI1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
SMILES stringCOC(=O)c1ccccc1C2c3ccc(N)cc3Oc4cc(N)ccc24
storage temp.20C

Code
Description
Unit Size
List Price
Qty
D1054-2MG
Unit:2MG
List Price: £71.40
Source:List Price
ADD
Cas Number109244-58-8