D0632-5G Display Image
D0632-5G Display Image

DL-Dithiothreitol,;, >=98% (TLC), >=99.0% (titration)

Code: D0632-5G D2-231

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulf...

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£129.00 5G
List Price

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.

Packaging

1 kg in poly drum

1, 5, 10, 25, 100 g in poly bottle

Quality Level200
storage temp.2-8C
InChI keyVHJLVAABSRFDPM-QWWZWVQMSA-N
assay99.0% (titration)
assay98% (HPLC)
SMILES stringO[C@H](CS)[C@H](O)CS
solubilityH2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
InChI1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
mp41-44 C (lit.)

Code
Description
Unit Size
List Price
Qty
D0632-1G
Unit:1G
List Price: £34.70
Source:List Price
ADD
D0632-10G
Unit:10G
List Price: £254.00
Source:List Price
ADD
D0632-100G
Unit:100G
List Price: £1,360.00
Source:List Price
ADD
D0632-25G
Unit:25G
List Price: £491.00
Source:List Price
ADD
Cas Number3483-12-3