An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
1 kg in poly drum
1, 5, 10, 25, 100 g in poly bottle