Application

Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse is suitable for the following applications:Immunohistochemistry (formalin-fixed, paraffin-embedded sections) using protease-digested sections of human or animal tissues.Immunohistochemistry (frozen sections).Indirect immunofluorescence (at a working dilution of 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues).Immunocytochemical labeling (immunofluorescence ) of cells.Western blotting.

Biochem/physiol Actions

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in the epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Cytokeratin, pan (mouse IgG1 and IgG2a isotypes) is a mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS1A3, M20, and A53-B/A2. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1 (68 kDa), 4 (59 kDa), 5 (58 kDa), 8 (52 kDa), and 6 (56 kDa) are members of the type II neutral-to-basic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia, stratified epithelia, simple epithelia and in tissues with natural or pathological turnover respectively. Cytokeratins 10 (56 kDa), 13 (54 kDa, and 18 (45 kDa) are members of the type I acidic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia and simple epithelial cells respectively. Cytokeratin peptide 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites.

Immunogen

mixture of several monoclonal cytokeratin clones.

Specificity

This is a mixture of monoclonal anti-cytokeratin antibodies. It recognizes human cytokeratins 1,4,5,6,8,10,13,18, and 19. It is a broad spectrum reagent, which reacts specifically with a wide variety of normal, reactive, and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Increased staining intensity is seen following proteolytic treatment (protease unmasking).