BIO27027 Display Image

Bioline Quick-Stick Ligase - 50 Reactions

Code: BIO27027 24

Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to impr...

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£99.17 EACH List Price
Discontinued

Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5’-phosphate and the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration. Quick-Stick Ligase will ligate 99% of ?/HindIII cohesive-ended fragments, or 80% of ?/EcoRV blunt-ended fragments, in 5 minutes at room temperature. 100% ligation of blunt-ended fragments can be achieved by increasing the ligation time to 15 minutes at room temperature.

Features

  • Dramatically decreases time for DNA cloning
  • Rapid 5 to 15 minute protocol at room temperature
  • Efficient and reliable ligations of cohesive and blunt-ended DNA
  • No loss of transformation efficiency

Applications

  • Cloning of DNA from:PCR products, Plasmids, Cosmids, Genomic, phage and viral DNA
  • Linker ligation
  • Re-ligation of linearized plasmids
  • Ligation of double-stranded oligonucleotides into vectors (plasmid and phage)

Components: Quick-Stick Ligase, 4x QS BufferDNA, Dilution Buffer

Storage Conditions: Quick-Stick Ligase can be stored for 12 months at -20°C.