RANGER DNA Polymerase is a hot-start enzyme, possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, thus offering both high-fidelity and enhanced specificity. The polymerase is supplied with an industry-leading novel buffer system, specifically formulated and validated to the unique properties of RANGER. RANGER is an easy-to-use high-performance enzyme specifically designed to amplify templates of 10kb upwards with extreme sensitivity . Owing to its antibody-based hot-start property, RANGER DNA Polymerase reactions can be setup at room temperature and has the added advantage of avoiding unwanted non-specific amplification and primer-dimer formation. This new hot-start enzyme from Meridian Bioscience is supplied with 5x RANGER Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, delivering superior amplification. RANGER is highly suitable for all PCR applications of long templates, including targeted re-sequencing using next-generation sequencers.

Features

• Long range, from 10kb-25kb
• Higher-fidelity than Taq, to ensure low error rates
• Validated for human genomic DNA
• Novel buffer system, including ultra-pure dNTPs and MgCl2
• Available as a convenient all-in-one master mix
• Applications: validated for human genomic DNA up to 25kb, suitable for TA cloning
• Components: RANGER DNA Polymerase, 5x RANGER Reaction Buffer
• Concentration: 4u/µl Volume BIO-21121: 62 x 50µl Reactions: 1 x 62.50µl BIO-21122: 125 x 50µl Reactions: 1 x 125µl BIO-21123: 625 x 50µl Reactions: 2 x 312.50µl
• Storage Conditions: RANGER DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided
• Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C