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Bioline RANGER DNA Polymerase - 250 Units

Code: BIO21121 24

RANGER DNA Polymerase is a hot-start enzyme, possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, thus offering both high-fidelity and enhanced specificity. The polymerase...

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RANGER DNA Polymerase is a hot-start enzyme, possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, thus offering both high-fidelity and enhanced specificity. The polymerase is supplied with an industry-leading novel buffer system, specifically formulated and validated to the unique properties of RANGER. RANGER is an easy-to-use high-performance enzyme specifically designed to amplify templates of 10kb upwards with extreme sensitivity . Owing to its antibody-based hot-start property, RANGER DNA Polymerase reactions can be setup at room temperature and has the added advantage of avoiding unwanted non-specific amplification and primer-dimer formation. This new hot-start enzyme from Bioline is supplied with 5x RANGER Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, delivering superior amplification. RANGER is highly suitable for all PCR applications of long templates, including targeted re-sequencing using next-generation sequencers.

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.

Features

  • Long range, from 10kb-25kb
  • Higher-fidelity than Taq, to ensure low error rates
  • Validated for human genomic DNA
  • Novel buffer system, including ultra-pure dNTPs and MgCl2
  • Available as a convenient all-in-one master mix

Applications

  • Validated for human genomic DNA up to 25kb
  • Suitable for TA cloning

Components

RANGER DNA Polymerase, 5x RANGER Reaction Buffer

Concentration: 4u/µlVolume BIO-21121: 62 x 50µl Reactions: 1 x 62.50µl BIO-21122: 125 x 50µl Reactions: 1 x 125µl BIO-21123: 625 x 50µl Reactions: 2 x 312.50µl

Storage Conditions: RANGER DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.

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BIO21122
Bioline RANGER DNA Polymerase - 500 Units
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