MyFi™ is a newly developed, antibody-mediated, hot-start enzyme with unique properties, that offers 3.5x higher fidelity than native Taq and enhanced sensitivity and specificity, making MyFi a superior choice for cloning. The polymerase is supplied with MyFi Buffer, a highly optimized proprietary formulation, containing ultra-pure dNTPs, MgCl2 and enhancers, specifically formulated and validated to provide outstanding results. MyFi is ideally suited for difficult PCR amplification and cloning of targets with variable lengths up to 10kb, for example, amplification of cDNA libraries, complex genomic fragments , targets with high GC-content and low-copy assays which require both high processivity and higher fidelity. MyFi has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation. MyFi generates new products with 3'-A overhangs, perfect for TA cloning. Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
Features
- Higher-fidelity, specifically developed for TA cloning
- Novel antibody-based hot-start polymerase
- Amplifies genomic DNA fragments up to 10kb
- Industry-leading novel buffer system
- Available as an all-in-one master mix
Applications
- Specifically developed for TA cloning PCR requiring high specificity combined with higher fidelity
- Good for low copy PCR assays
Components
MyFi DNA Polymerase, 5x MyFi Reaction Buffer
Concentration: 2u/µl Volume BIO-21117: 125 x 25µl Reactions: 1 x 125µl BIO-21118: 250 x 25µl Reactions: 1 x 250µl BIO-21119: 1250 x 25µl Reactions: 2 x 625µl
Storage Conditions: MyFi DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
This product has met the following criteria: