MyTaq™ Red DNA Polymerase is a high-performance PCR product that exhibits more robust amplification than other commonly used polymerases . MyTaq Red DNA Polymerase delivers very high yield over a wide range of PCR templates, making MyTaq Red DNA Polymerase the ideal choice for most routine assays. This new enzyme preparation from Meridian Bioscience is supplied with a 5x MyTaq Red Reaction Buffer system, a proprietary formulation that saves time and delivers superior results. MyTaq buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations that eliminate the need for further optimization. The specially designed MyTaq Red formulation does not interfere with PCR reactions and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose). MyTaq Red only requires the addition of template, primers and water, thus reducing the risk of pipetting errors and contamination, as well as shortening the set-up time.
Features
- New generation polymerase with superior performance
- Increased sensitivity and speed
- Novel buffer system, including dNTPs and MgCl2
- Robust and high yield across a wide range of templates
- Includes red dye for direct gel loading
- Applications: high-throughput PCR, specific amplification of complex templates, robust amplification of GC-rich sequences, TA cloning
- Components: MyTaq DNA Polymerase, 5x MyTaq Reaction Buffer
- Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C
- Concentration: 5u/µlStorage Conditions: MyTaq Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
This product has met the following criteria: