Application

Anti-Bovine IgG (whole molecule) antibody produced in rabbit is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000.

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used to coat immulon 4HBX platesin serum antibody enzyme-linked immunosorbent assay (ELISA) and western blotting.

Biochem/physiol Actions

Immunoglobulin G (IgG) initiatesthe classical pathway of the complementsystem and also nullifies virus particles and toxins.Maternal IgG is transferred to the fetus through the placenta that iscriticalfor the immune defense of the neonate against infections. IgG modulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associatedwith allergy. Digestion of IgG by papain releasesfragment antigen-binding (Fab) and pepsin digestion of IgGproduces crystallizable (fc)fragmentand a single dimeric F(ab)₂.IgGlevels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patientsmay be used toevaluatethe severity and prognosis of coronavirus disease 2019 (COVID-19).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is the most importanttype of glycoprotein antibodypresentinhuman serum. It is found inblood and hasfour polypeptide chains.Thelight and heavychains are interconnected by disulfide bonds. IgG has the longest serum half-life when compared to other immunoglobulins.

IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum proteins. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.