Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Components

This product contains: α₂-macroglobulin (equine serum) β-galactosidase (E. coli) phosphorylase b (rabbit muscle) catalase (bovine liver) alcohol dehydrogenase (yeast) carbonic anhydrase (bovine erythrocyte) trypsin inhibitor (soy bean) lysozyme (chicken egg) aprotinin (bovine lung)Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Other Notes

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitution

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.