Application

Monoclonal Anti-Phosphotyrosine?Biotin antibody produced in mouse has been used in western blot analysis to detect tyrosine phosphorylated proteins. It has also been used in BIAcore analysis. This conjugate maybe used as an analytical tool by enabling the identification and quantification of tyrosine phosphorylated proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biotin antibody can be used in dot blot (diluted 1:32,000). Mouse anti-phosphotyrosine?biotin antibody reacts specifically with phosphorylated tyrosine both as the free amino acid or when conjugated to BSA or KLH. This product does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine and threonine, or with phosphorylated molecules like AMP or ATP.

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.

Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

phosphotyrosine conjugated to BSA

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.