Analysis Note

ControlHeLa acid extract and recombinant histone proteins

Application

Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.

Use Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.

General description

Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A's lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in HeLa acid extract and recombinant histone proteins.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.

Specificity

This antibody recognizes Histone H2A at the C-terminus.

Broad species reactivity expected based on sequence similarity.

Target description

~16 kDa observed