Application

Research CategoryEpigenetics & Nuclear Function

Anti-Nek6, Cat. No. ABE2875, is a highly specific rabbit polyclonal antibody that targets Serine/threonine-protein kinase Nek6 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nek6 in human pancreas and human cervical cancer tissue.

Immunofluorescence Analysis: A representative lot detected Nek6 in HeLa cells that were transfected with Nek6 siRNA (O′Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90).

Western Blotting Analysis: A representative lot detected Nek6 in IP blot from lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole, HeLa cells were either mock transfected or transfected (O′Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O′Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).

Immunoprecipitation Analysis: A representative lot detected Nek6 in lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole (O′Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O′Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Serine/threonine-protein kinase Nek6 (UniProt: Q9HC98; also known as EC: 2.7.11.1, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512) is encoded by the NEK6 gene (Gene ID: 10783) in human. Nek6, a member of the NIMA-related serine/threonine kinase family that plays an important role in mitotic cell cycle progression and is required for chromosome segregation at metaphase-anaphase transition. It localizes to spindle microtubules in metaphase and anaphase and to the mid-body during cytokinesis. Nek6 is ubiquitously expressed, with highest expression in heart and skeletal muscle. Its levels are up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. Nek6 is shown to be directly phosphorylated by Chk1 and Chk2, suggesting that it is a direct target of the DNA damage checkpoint. It is also phosphorylated upon IR or UV-induced DNA damage. Nek6 is activated by phosphorylation at Ser206. Although expression of Nek6 is tightly controlled in normal cells, this protein is highly overexpressed in various human cancers and regulates tumor progression and its expression and activity levels directly correlate to tumorigenicity of human cancer cells. Inhibition of Nek6 can lead to apoptosis. (Ref.: Nassirpour, R et al. (2010). Mol. Cancer Res. 8(5); 717-28).

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human Serine/threonine-protein kinase Nek6.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in HT1080 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nek6 in 10 µg of HT1080 cell lysate.

Specificity

This rabbit polyclonal antibody detects Serine/threonine-protein kinase Nek6 in human cells. It targets an epitope with in the first 15 amino acids in the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed; 35.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).