Application

Anti-NR5A2 (LRH1), Cat. No. ABE2867, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Chromatin Immunoprecipitation Analysis: A representative lot detected NR5A2 (LRH1) in Chromatin Immunoprecipitation applications (Kaltezioti, V., et. al. (2010). PLoS Biol. 8(12):e1000565; Venteclef, N., et. al. (2010). Genes Dev. 24(4):381-95; Boulias, K., et. al. (2005). EMBO J. 24(14):2624-33; Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105).

Western Blotting Analysis: A representative lot detected NR5A2 (LRH1) in Western Blotting applications (Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105; Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

Immunofluorescence Analysis: A representative lot detected NR5A2 (LRH1) in Immunofluorescence YES titration paraffin sections from E10.5 (a), E12.5 (b), E14.5 (c) or E16.5 (d) mouse spinal cord (Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5′-AACGACCGACCTTGAG-3′ of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).

Immunogen

His-tagged full length recombinant human Nuclear receptor subfamily 5 group A member 2, isoform 2.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antiserum with 0.05% sodium azide.

Format: Unpurified

Unpurified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NR5A2 (LRH1) in 10 µg of HEK293 cell lysate.

Specificity

This rabbit polyclonal antibody detects Nuclear receptor subfamily 5 group A member 2 (NR5A2) in human and murine cells.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~68 kDa observed; 61.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).