Analysis Note

ControlRAW264.7 cell lysate

Application

Anti-MILI Antibody is a Rabbit Polyclonal Antibody for detection of MILI also known as Piwi-like protein 2 & has been validated in WB, ICC, IHC & IP.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in male primordial germ cells. (Aravin, A.A., et al. (2008). Mol Cell. 31(6):785-799.)

Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MILI, MIWI, and MIWI2 belong to the Piwi family of proteins. piRNAs and Piwi proteins are implicated in transposon control and are linked to transposon methylation in mammals. MILI, in particular, directly binds with piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. MILI plays an essential role in spermatogenesis, meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.

Immunogen

KLH-conjugated linear peptide corresponding to mouse MILI.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~109 kDa observed. An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.