Analysis Note

ControlRecombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts

Application

Research Sub CategoryHistones

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl-Histone H2B (Lys24) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-acetyl-Histone H2B (Lys24) (1:500 dilution).

Research CategoryEpigenetics & Nuclear Function

Use Anti-acetyl-Histone H2B (Lys24) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP, DB to detect acetyl-Histone H2B (Lys24) also known as H2BK24Ac, Histone H2B (acetyl K24).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

Immunogen

Epitope: Acetylated Lys24

KLH-conjugated linear peptide corresponding to human Histone H2B acetylated at Lys24.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.

Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H2B on 10 µg of recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.

Specificity

This antibody recognizes Histone H2B acetylated at Lys24.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed