Application

Immunofluorescence Analysis: A representative lot detected SYCP2 in spread nuclei of mouse spermatocytes. (Courtesy of Dr. P. Jeremy Wang at University of Pennsylvania).

Immunofluorescence Analysis: A representative lot detected SYCP2 in spread nuclei of oocytes and spermocytes (Yang, F., et. al. (2015). EMBO Mol Med. 7(9):1198-210).

Western Blotting Analysis: A representative lot detected SYCP2 in adult mouse testis tissue lysate (Yang, F., et. al. (2006). J Cell Biol. 173(4):497-507).

Research CategoryEpigenetics & Nuclear Function

Anti-SYCP2 , Cat. No. ABE2622, is a highly specific rabbit polyclonal antibody that targets Synaptonemal complex protein 2 and has been tested in Immunofluorescence and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptonemal complex protein 2 (UniProt: Q9CUU3; also known as SCP-2, SYCP2, Synaptonemal complex lateral element protein) is encoded by the Sycp2 (also known as Scp2) gene (Gene ID: 320558) in murine species.SYCP2 is present in testis and spermatocyte and is a major component of the axial/lateral elements of synaptonemal complexes (SCS) during meiotic prophase and plays a role in the assembly of synaptonemal complexes. It is required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility. SYCP2 is considered to be a primary determinant of axial/lateral elements and can heterodimerize with SYCP3 and it is is required for the incorporation of SYCP3 into the synaptonemal complex. An evolutionarily conserved coiled coil domain in SYCP2 is required for binding to SYCP3. Defects in SYCP2 leads to sterility in male mice due to defective meiotic chromosome synapsis during spermatocyte development. Also, SYCP2 deficient mice show much reduced testes weight.

Immunogen

Epitope: C-terminus

His-tagged recombinant fragment corresponding to 246 amino acids from the C-terminal region of murine Synaptonemal complex protein 2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal anti-serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SYCP2 in 50 µg of mouse testis tissue lysate.

Specificity

This polyclonal antibody detects Synaptonemal complex protein 2 in mouse testis and recognizes an epitope within amino acids 1255-1500 in the C-terminal region.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~200 kDa observed; 172.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).