Application

ChIP-seq Analysis: A representative lot detected BMAL1 in Beta-TC6 cell DNA used for chip assays (Perelis, M., et. al. (2015). Science. 350(6261):aac4250).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected BMAL1 in Beta-TC6 cell DNA used for chip assays (Perelis, M., et. al. (2015). Science. 350(6261):aac4250).

Research CategoryEpigenetics & Nuclear Function

Anti-BMAL1, Cat. No. ABE2599, is a highly specific guinea pig polyclonal antibody that targets Aryl hydrocarbon receptor nuclear translocator-like protein 1 and has been tested for use in and Chromatin Immunoprecipitation (ChIP), ChIP-seq, and Immunoprecipitation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: Q9WTL8; also known as Arnt3, Brain and muscle ARNT-like 1, BMAL1) is encoded by the Arnt1 (also known as Bmal1) gene (Gene ID: 11865) in murine species. BMAL1 is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart. It shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription, and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and premature aging.

Immunogen

KLH-conjugated linear peptide corresponding to 37 amino acids from the N-terminal half of murine Aryl hydrocarbon receptor nuclear translocator-like protein 1 (BMAL1). This sequence is conserved in isoforms 2, 4, and 5.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity Purified

Format: Purified

Purified guinea pig polyclonal antibody in PBS with 0.05% sodium azide.

Quality

Evaluated by Immunoprecipitation in HeLa cell nuclear extract.

Immunoprecipitation Analysis: 5 µg of this antibody detected BMAL1 in HeLa cell nuclear extract.

Specificity

This guinea-pig polyclonal antibody detects Aryl hydrocarbon receptor nuclear translocator-like protein 1 (BMAL1). It targets an epitope within 37 amino acids from the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed. Uncharacterized bands may be observed in some lysate(s).