Analysis Note

ControlRAW 264.7 cell lysate

Application

Anti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB.

Research Sub CategoryHormones & Receptors

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.

Immunogen

KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.

Linkage

Replaces: 06-892

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in RAW 264.7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa