Analysis Note

ControlNIH/3T3 cell lysate

Application

This rabbit polyclonal Anti-BPTF Antibody has been published and validated for use in Immunoprecipitation and Western Blotting applications.

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected BPTF in 50 µg of human HepG2 cell lysate. BPTF shRNA treatment greatly reduced cellular BPTF level (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected BPTF in 50 µg of wild-type, but not Bptf-knockout, mouse embryonic fibroblast (MEF) lysate (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Ctcf and SA2 with BPTF from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).

Western Blotting Analysis: A representative lot detected BPTF in Ctcf and SA2 immunoprecipitates from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).

Western Blotting Analysis: A representative lot detected BPTF in ESC, MEF, and CD8+/CD4+ double-positive (DP) thymocytes from wild-type, but not Bptf-knockout, mice (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).

Note: ABE24 is not recommended for Chromatin Immunoprecipitation (ChIP). For ChIP application, please use ABE1966.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleosome-remodeling factor subunit BPTF (UniProt A2A654 and E9Q6A7; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen) is encoded by the Bptf (also known as Fac1, Falz) gene (Gene ID 207165) in murine species. BPTF, SNF2L and pRBAP46/48 are the three subunits of the nucleosome-remodelling factor NURF, an ISWI chromatin-remodeling complex that catalyzes ATP-dependent nucleosome sliding. BPTF mediates NURF target site specificity via interactions with transcription factors, histone variants and histone modifications on transcriptionally active genes. BPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. BPTF is necessary for the survival of c-MYC-overexpressing cells and for c-MYC-driven tumorigenesis in the mouse pancreas. BPTF knockdown leads to decreased c-MYC chromatin accessibility and significantly delays tumour development in pre-neoplastic pancreatic acinar cells. Consistently, BPTF expression in human tumours positively correlates with activation of c-MYC gene signatures.

Immunogen

GST-tagged recombinant mouse BPTF N-terminal fragment.

Epitope: N-terminal region.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected BPTF in 10 µg of NIH/3T3 cell lysate.

Specificity

This rabbit polyclonal antibody detected BPTF in wild-type, but not Bptf-knockout mouse cell lysates. A greatly reduced target band was observed using lysates from BPTF shRNA-treated human HepG2 cells (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237; Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~300/250/100 kDa observed. 333.2/321.6 kDa (mouse; UniProt A2A654/E9Q6A7) and 338.3/325.1/322.2 kDa (human isoform 1/2/3; UniProt Q12830) calculated.