Analysis Note

ControlHeLa cell lysate

Application

Use Anti-Gdown1 Antibody (Sheep Polyclonal Antibody) validated in WB, ChIP to detect Gdown1 also known as Myocardial zonula adherens protein, GRINL1A upstream protein, Gup.

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gdown1 (DNA-directed RNA polymerase II subunit GRINL1A; DNA-directed RNA polymerase II subunit M; glutamate receptor-like protein 1A) is a nuclear protein belonging to the GRINL1 family. It binds to the RNA Polymerase II (G) molecule (RNAPII (G)) and prevents its interaction with transactivating factors, thereby suppressing RNAPII (G)-mediated transcriptional events. Gdown1 also indirectly suppresses RNAPII (G)-transcription by blocking the binding of the elongation-enhancing factor TFIIF to the elongation complex. Gdown1 also prevents TFIIF-mediated assembly of the preinitiation complex required for recruiting RNAPII to the promoter region, by binding competitively to Pol II at the RPB1 and RPB5 subunits recognized by TFIIF. Gdown1 may also inhibit the termination factor TTF2. The effects of Gdown1 are blocked by the P-TEFb. Overall, Gdown1 regulates the transcriptional activity of RNAPII (G) at multiple promoter regions by various mechanisms. Gdown1 has been detected in brain, testis, prostate, lung, small intestine, heart, kidney, and skeletal muscle.

Immunogen

Histidine-tagged recombinant protein corresponding to human Gdown1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified sheep polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed. Uniprot describes 11 isoforms ranging between 44 and 64 kDa