Analysis Note
ControlHepG2 cell lysate
Application
Research Sub CategoryTranscription Factors
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Smad4 in NIH/3T3, HeLa, and A431 cells.
Use Anti-Smad4/DPC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Smad4/DPC4 also known as Mothers against decapentaplegic homolog 4, SMAD 4, Smad4, hSMAD4.
Research CategoryEpigenetics & Nuclear Function
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors.
Immunogen
GST-tagged recombinant protein corresponding to human Smad4 at the SAD region.
Epitope: SAD region
Linkage
Replaces: 04-1033
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Smad4 on 10 µg of HepG2 cell lysate.
Specificity
This antibody recognizes Smad4 at the SAD region.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~68 kDa observed.
This product has met the following criteria: