Application

This Anti-TERT Antibody is validated for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of TERT.

Immunocytochemistry Analysis: An 1:200 dilution from a representative lot detected TERT in A431, HeLa, HUVEC and NIH/3T3 cells.

Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected TERT in human cerebral cortex, human pancreas, and rat testis tissue sections.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Telomerase reverse transcriptase (EC 2.7.7.49; UniProt O14746; also known as hEST2, hTRT, Telomerase-associated protein 2, Telomerase catalytic subunit, TP2) is encoded by the TERT (also known as CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TRT) gene (Gene ID 7015) in human. Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase responsible for adding TTAGGG repeats to the chromosome telomere ends. Cells with low or no telomerase expression lose telomere repeats during cell division, eventually resulting in cellular senescence. Most cancer cells, germ cells and embryonic stem cells express high levels of telomerase, thus contributing to pluripotency and immortality. In addition to its telomere maintenance function, telomerase also has a pro-survival role in cellular resistance against DNA damage and ensuing apoptosis. Most cancer cells are highly proliferative and express high levels of nuclear telomerase activity. Studies show that TERT shuttles from the nucleus into mitochondria upon oxidative stress induction in cancer cells. TERT mitochondrial localization helps prevent nuclear DNA damage by decreasing mitochondrial reactive oxygen species (ROS), accounting for high stress resistance especially among the cancer stem cells (CSCs) population. Following exposure to H2O2 or gamma-irradiation, cancer cells capable of excluding TERT from the nucleus display little or no DNA damage, while TERT nuclear retainment results in high DNA damage.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence from the C-terminal extension (CTE) region of human TERT.

Epitope: C-terminal extension (CTE).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TERT in 10 µg of HeLa cell lysate.

Specificity

This polyclonal antibody targets a sequence toward the end of the C-terminal extension (CTE) region present in spliced isoforms 1 and 3, but not in isoforms 2 and 4 of hTERT reported by UniProt (O14746).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed. 127.0/120.0 kDa (human isoform 1/3) calculated.