Application

Use Anti-Sirt5 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Sirt5 also known as NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5.

Immunohistochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected Sirt5 in convoluted tubules of normal rat kidney tissue.

General description

Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.

Immunogen

KLH-conjugated linear peptide corresponding to human Sirt5.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Sirt5 on 10 µg of HeLa cell lysate.

Target description

~34 kDa observed. Two isoforms at ~33 and ~34 kDa are known to exist.