Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot immunostained centrisomes in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized U2OS human osteosarcoma cells (Courtesy of Tae-Sung Kim, Ph.D. and Kyung Lee, Ph.D., NCI, NIH, U.S.A.).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected in Cep152 in lysate from luciferase shRNA-transfected, but not Cep152 shRNA-transfected U2OS human osteosarcoma cells (Courtesy of Tae-Sung Kim, Ph.D. and Kyung Lee, Ph.D., NCI, NIH, U.S.A.).

Anti-Cep152 (P7), Cat. No. ABE1977, is a highly specific rabbit polyclonal antibody that targets Cep152 and has been tested in Immunocytochemistry and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Centrosomal protein of 152 kDa (UniProt O94986; also known as Cep152) is encoded by the CEP152 (also known as KIAA0912, MCPH9, SCKL5) gene (Gene ID 22995) in human. The mammalian Asl (Asterless) ortholog Cep152 is a centrosomal protein (CEP) that plays an important role in centriole duplication and centriole-to-centrosome conversion. The centrosome is the main microtubule-organizing center essential spindle formation and chromosome segregation during mitosis. Centrosomes are composed of two orthogonally arranged centrioles surrounded by pericentriolar material (PCM). Centriole duplication begins by assembling a procentriole in G1/S phase, where centrosomal scaffolds Cep152 and Cep192 (Spd-2 ortholog) mediate the recruitment of polo kinase Plk4 to to centrosomes, a key event that triggers the entire duplication process. New centrioles assemble perpendicularly to pre-existing ones in G1/S and elongate throughout S and G2. The conversion of fully elongated daughter centrioles into centrosomes during mitotic progression requires sequential loading of Cep135, Cep295 (Ana1 analog) and Cep152 onto daughter centrioles.Cep152 is also an important regulator of genomic integrity and cellular response to DNA damage. CEP152 gene mutations are identified among individuals with Seckel syndrome, a heterogeneous autosomal recessive disorder characterized by a proportionate short stature, severe microcephaly and mental retardation, and a typical ′bird-head′ facial appearance. Cep152 impairment in Seckel patients-derived cells leads to accumulation of genomic defects as a reuslt of replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.

Immunogen

Recombinant human Cep152 C-terminal fragment.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 80 mM Tris (pH 8.0), 40 mM glycine, and 50% ethylene glycol with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cep152 in 10 µg of U2OS human osteosarcoma cell lysate.

Specificity

This rabbit polyclonal antibody immunostains centrisomes by indirect fluorescence and detected shRNA-mediated Cep152 knockdown by Western blotting. Target C-terminal region is present in human Cep152 spliced isoforms 3 and 4, but more than 95% absent in spliced isoforms 1 and 2 reported by UniProt (O94986).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~200 kDa observed. 189.0/195.6 kDa (isoform 3/4) calculated. Uncharacterized bands may be observed in some lysate(s).