Application

Anti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

General description

Borealin (UniProt: Q53HL2; also known as Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein) is encoded by the CDCA8 (also known as PESCRG3) gene (Gene ID: 55143) in human. Borealin is a novel 31-kDa chromosomal passenger protein that is conserved among all vertebrates. It is a member of the chromosomal passenger holocomplex along with Aurora B, INCENP, and survivin. This complex play a vital role at the centromere to ensure proper chromosome alignment and segregation. Borealin is required for stability of the bipolar mitotic spindle and for kinetochore error correction. Borealin levels are reported to increase during G2/M phase and are reduced following exit from the M phase of cell cycle. Depletion of Borealin by RNAi is shown to retard mitotic progression and cause spindle T misattachment and abnormalities. Borealin-depleted cells are shown to align their chromosomes to a near normal metaphase plate, but they abruptly undergo multipolar anaphases.

Immunogen

A His-tagged full length human recombinant Borealin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in Hela cell lysate treated with Nocodozole.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.

Specificity

This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.

Target description

~36 kDa observed; 31.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).